Note that the whole fractionation range of superose 6 increase is not covered in this diagram. Filled with stationary phase compositions or resins, the prepacked options reduce the preparing time required. Superdex 75 10300 gl and superdex 200 10300 gl are tricorn high performance columns. Rapid purity check and screening superdex 75 increase 5150 gl, superdex 200 increase 5150 gl and superose 6 increase 5150 gl are short columns with small bed volumes that are suitable for rapid protein homogeneity analyses or purity checks. Highsample load 500 l separation of a mixture containing seven. Each is available in two column sizes, hiload 16600 and hiload 26600. Superdex 75 10300 gl from ge healthcare selectscience. Igg pdf structural characterization of vapb46 antitoxin from. The steep selectivity gives highresolution separations in the molecular weight range of 100 to 600 000. Superdex 30, 75 and 200 prep grade are preparative gel filtration media with a unique. Safety for use and handling of the products in a safe way, refer to the safety data sheet. Superdex 75 increase cytiva, formerly ge healthcare life.
Hiload 16600 and 26600 superdex 30 prep grade hiload 16. The separation properties of these media are determined by the dextran componentchromatography columns separate molecules within mixtures during gas or liquid methods. Sec columns for analysis and purification of peptides superdex 30 increase is suitable for both preparative and analytical purposes. Selectivity curves from superdex 30 pg, superdex 75 pg and superdex 200 pg. The columns are intended for use in preparative purification as well as for characterization and analysis of proteins with molecular weights between m r 3000 and 70 000, such as recombinant tagged proteins. The columns are prepacked glass columns for high performance gel filtration of proteins, peptides, dna fragments superdex 75 increase is designed for smallscale preparative purification and analysis of recombinant tagged or other proteins with molecular weights from 3000 up to 70 000, such as recombinant tagged proteins features and benefits comprising smaller, more rigid beads. Superdex gel filtration media are listed in appendix c, table 1. Superdex prep grade and prepacked hiload columns 1 chromatography media characteristics 2 chemical stability and dextran. Superdex 75 increase belongs to the new generation of sec columns that replaces the wellknown superdex 75 columns. Superdex prepacked columns fig 1 are designed for highperformance, laboratoryscale separations of proteins, peptides, and other biomolecules according to size. Superdex 75 10300 gl ge healthcare, 17517401, column l. Selectivity curves from superdex 30 prep grade, superdex 75 prep grade, and. Flow rate limits and recommendations superdex 75 increase 10300 gl note. Superdex 5150 gl and 10300 gl prepacked tricorn columns.
Hiload 16600 and 26600 superdex 200 prep grade introduction hiload 16600 and 26600 superdex 30 prep grade, hiload 16600 and 26600 superdex 75 prep grade, and hiload 16600 and 26600 superdex 200 prep grade pg are prepacked xk columns designed for preparative gel filtration. Every effort has been made to ensure the accuracy of the superdex 50% information published above. Superdex 75 increase 5150 gl an d superdex 75 increase 10300 gl columns are intended for research use only, and shall not be used in any clinical or in vitro procedures for diagnostic purposes. Ge healthcare superdex 200 increase 10300 gl prepacked tricorn columns for size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000.
Superdex 200 10300 gl chromatographic separation column. As shown in figures 4 a and b, the detection signal from the run with superdex 200 increase 3. Highresolution analysis and purification of small sample volumes, especially when sample amount is limited and low buffer consumption is important. Ge healthcare size exclusion chromatography superdex 30. Superdex prep grade is a matrix of dextran and highly crosslinked agarose. New superdex 200 increase columns fisher scientific. For olecules with molecular weights between 0 and 600000 da. Data file 18116379 ad gel filtration superdex highperformance columns fig 1.
Ge healthcare superdex 75 increase prepacked columns are ideal when time is limited and low buffer and sample consumption is important. Instructions 56119099 ae gel filtration media superdex. The high molecular weight kit contains 5 proteins with molecular weights in the range 44 000 to 669 000 and blue dextran 2000. Size exclusion chromatography columns and media fisher scientific. Superdex 75 increase 5150 gl, superdex 200 increase 5150 gl and superose 6 increase 5150 gl are short columns with small bed volumes that are suitable. Is it possible to rescue, to some degree, a compressed gel filtration column superdex 75 or 200 1030 gl. Superdex 200 increase smallscale sec columns cytiva. Ge healthcare superdex 75 increase prepacked column, 5150. General description superdex 75 10300 gl is a prepacked gel filtration column for highresolution, semipreparative, and analytical separations of biomolecules. Superdex 30 prep grade medium is optimized for proteinspeptides below m r 10 000. Superdex 200 increase prepacked columns deliver up to 50% higher resolution in less time than the predecessors superdex 200.
The steep selectivity of the dextran component and the high. Data file 29045269aa size exclusion chromatography superdex 200 increase columns superdex 200 increase prepacked columns fig 1 are designed for size exclusion chromatography sechigh resolution gel filtration in smallscale g to mg, preparative purification, as well as for characterization and analysis. For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. Superdex 75 increase 10300 gl is suitable for both smallscale preparative purification and for analytical applications. With their high physical and chemical stability, very high batchtobatch reproducibility, and regulatory support file backup, superdex 30 prep grade, superdex 75 prep grade and superdex 200 prep grade are ideal for all stages of an industrial scale operation from research and. Superdex 75 prep grade and superdex 200 prep grade are ideal for all stages. Instructions 56119099 ae gel filtration media superdex 30. The columns are prepacked glass columns for high performance gel filtration of proteins, peptides, dna fragments superdex 75 increase 10300 gl is suitable for both smallscale preparative purification and for analytical applications.
Selectivity curves for superdex peptide, 75, and 200. Data sources include ibm watson micromedex updated 10 apr 2020, cerner multum updated 6 apr 2020, wolters kluwer updated. Chemical, physical and chromatographic properties of. Chemical, physical and chromatographic properties of superdex. Rapid purity check and screening superdex 75 5150 gl, superdex 200 increase 5150 gl and superose 6 increase 5150 gl are short columns with small bed volumes that are suitable for rapid protein homogeneity analyses or purity checks. The steep selectivity of the dextran component 2 nonspecific interaction 3 column characteristics 4 operation 4 optimization 4 applications 5 hiload superdex 30 pg 5 hiload superdex 75 pg 6 hiload superdex 200 pg 6.
Hiload superdex 30 prep grade hiload superdex 75 prep grade hiload superdex 200 prep grade hiload columns are xk laboratory columns prepacked with superdex prep grade for gel filtration. Superdex prep grade is a composite matr ix of dextran and. Protein purification chromatography calibration of. Promo code superdex 200 increase 10300 gl suitable for both smallscale mg, preparative and highresolution analysis applications 28990944 45002570 45002570pr superdex 200 increase 5150 gl designed for rapid purity checking and quick screening of different. Superdex 75 5150 gl and superdex 200 5150 gl are short columns with small bed volumes that are suitable for rapid protein homogeneity analyses or purity checks. Use columns prepacked with superdex 30 prep grade, superdex 75 prep grade. Ge healthcare lifescience superdex 200 increase 10300 gl prepacked tricorn columns are designed for small scale preparative purification. Superdex 30 increase is part of ges new generation of agarosebased sec columns called increase, which offer higher resolution purification in shorter runtimes compared with their predecessors. Why the column pressure of superdex 75 10300 gl has. Separation of test substances on hiload 26600 superdex 30 pg.
Superdex 75 increase 5150 gl is for rapid size analysis of proteins and other biomolecules. Equilibration of the column must be done with 2 bed volumes of the equilibration buffer before any experiment is. Steep selectivity curves give gppd resolving power for peptides and proteins in the molecular weight range mr 10 000 600 000 superdex 200 pg. Superdex 200 increase from, your source for laboratory equipment and scientific supplies. However, it remains the responsibility of the readers to familiarize themselves with the product information contained on the canadian product label or package insert. Journal of chromatography, 537 1991 1732 elsevier science publishers b. Hiload superdex columns, 200 pg, ge healthcare vwr.
Protein analysis with size exclusion chromatography sec. Journal of chromatography, 547 1991 2 elsevier science publishers b. Superdex prepacked columns are designed for highperformance, laboratoryscale separations of proteins, peptides, and other biomolecules according to size. Superdex 30 increase ges newest sec column lab equipment. We are using superdex increase 10300 gl size exclusion column in our lab and recently column pre column pressure is increased and flow rate is low around 0. Hiload superdex 75 prep grade data file hiload superdex 200. Hiload 16600 superdex 75 pg cytiva formerly ge healthcare. Column data matrix dextran covalently bound to highly crosslinked agarose mean particle size 34. Size exclusion chromatography columns and media selection guide. Rapid purity check and screening superdex 75 5150 gl, superdex 200 increase 5150 gl and superose 6 increase 5150 gl are short columns with small bed. Stability of superdex 75 prep grade and superdex 200 prep. Safety for use and handling of the product in a safe way, refer to the safety data sheet. Ge healthcare size exclusion chromatography superdex 30 increase columns.
Jun 12, 2017 superdex 30 increase is part of ges new generation of agarosebased sec columns called increase, which offer higher resolution purification in shorter runtimes compared with their predecessors. Extracted from size exclusion chromatography principles and methods, pdf. Ge healthcare size exclusion chromatography superdex 75. Ge healthcare superdex 75 increase prepacked column, 5150 gl. Short cycle time, together with small sample volume and low. Protein purification chromatography calibration of hiload. Superdex 75 10300 gl prepacked column for smallscale preparative purification g to mg or characterization of proteins in the molecular range 3000 to 70 000, like recombinant, tagged proteins. The columns are intended for use in preparative purification as well as for characterization and analysis of proteins with molecular weights between m r 3000 and 70 000, such as recombinant tagged proteins up to 50% higher resolution compared with.
Most waterbased buffers can be considered to be similar to water, for example phosphate and tris buffers. Hiload superdex 75 prep grade separates proteins and peptides in the molecular weight range 3 00070 000 daltons and performs best between 8 000 and 50 000, which is ideal for recombinant proteins. Superdex 30, 75, and 200 prep grade media are available prepacked in hiload 1660 and hiload 2660 columns. Why the column pressure of superdex 75 10300 gl has increased to 1mpa even at a flow rate of 0. They save time when screening many samples, and require less buffer and sample than longer columns. Superdex prep grade pg is produced by covalent bonding of dextran to highly crosslinked agarose. Our new generation columns called increase offer higher resolution and shorter run times than their predecessors. Superdex 75 increase cytiva, formerly ge healthcare life sciences. Superdex 75 has a separation range for molecules with molecular weights between 3 000 and 70 000. Ge healthcare superdex 200 increase 10300 gl prepacked. The maximum column pressure for the superdex 200 column is 218 psi, and the recommended flow rate is 0. Pdf legumain expression, activity and secretion are.
Hiload superdex 75 pg prepacked columns are for highresolution size exclusion chromatography of recombinant proteins from samples up to 5 ml or ml. The main characteristics of superdex media superdex. This material is provided for educational purposes only and is not intended for medical advice, diagnosis or treatment. Superdex 75 increase belongs to the new generation of agarose based sec media, comprising smaller, more rigid beads with a narrower particle size distribution. Hiload superdex 30, 75, and 200 prep grade columns bring convenience and high resolution to gel filtration. Superdex prepacked columns are designed for highperformance, laboratoryscale separations of proteins, peptides, and other. Hiload superdex 30 prep grade is optimized for proteins and peptides, but it can also be used with success to separate oligosaccharides. Ge healthcare hiload 16600 superdex 200 pg hiload 1660. Introduction two gel filtration calibration kits are available for protein molecular weight determination by gel filtration. New superdex 200 increase columns product description ge cat. Superdex 30 prep grade, superdex 75 prep grade, and. Superdex 75 has a separation range for molecules with molecular weights between 3,000 and 70,000. Start with superdex 200 increase when the molecular weight of the protein of. Superdex 75 increase is designed for smallscale preparative purification and analysis of recombinant tagged or other proteins with molecular weights from 3000 up to 70 000, such as recombinant tagged proteins.
Use this column for rapid purity check and screening of proteins with molecular weights between mr 3,000 and 70,000, such as recombinant tagged proteins. Hiload superdex 75 prep grade data file hiload superdex. Dextran component determined the separation properties of these media. Buffers and solvents with increased viscosity will affect the backpressure and flow rate. Superdex 75 10300 gl is a prepacked gel filtration column for highresolution, semipreparative, and analytical separations of biomolecules. Instruction 29163059 ae size exclusion chromatography columns. Data file 18116379 ad gel filtration superdex high.
The low molecular weight kit contains 5 proteins with molecular weights in the range 6500 to 75 000 and blue dextran 2000. Hiload superdex 75 pg columns m r 3 00070 000 hiload superdex 30 pg columns m r 1660 superdex 200 prep grade is a prepacked xk column for preparative gel filtration chromatography separations. So if people are dong a manual clean it can easily happen that system will go over pressure limit of. Superdex 200 increase size exclusion chromatography columns are well suited for highresolution analysis and smallscale purification of monoclonal antibodies mab and other biomolecules with m r 10 000 to 600 000. Calibration of hiload 2660 superdex 200 prep grade. Quick information superdex 75 increase 5150 gl an d.
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